Regulatory

Part:BBa_K3930006:Design

Designed by: Thomas Gaudin   Group: iGEM21_Toulouse_INSA-UPS   (2021-10-07)


Inducer Z3EV of the estradiol promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1021
    Illegal XbaI site found at 1324
    Illegal PstI site found at 666
    Illegal PstI site found at 837
    Illegal PstI site found at 1340
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 666
    Illegal PstI site found at 837
    Illegal PstI site found at 1340
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1021
    Illegal XbaI site found at 1324
    Illegal PstI site found at 666
    Illegal PstI site found at 837
    Illegal PstI site found at 1340
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1021
    Illegal XbaI site found at 1324
    Illegal PstI site found at 666
    Illegal PstI site found at 837
    Illegal PstI site found at 1340
    Illegal AgeI site found at 1527
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1849
    Illegal BsaI.rc site found at 987


Design Notes

Source

This sequence has been synthetized by IDT, and is referenced in the article (Liu et al. 2020)

References